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This paper presents a serious game that simulates a water crisis triggered by the spill of an unregulated chemical. The game includes five stakeholder roles representing a chemical manufacturer, resident, water treatment plant, environmental agency, and health department, in addition to a facilitator role. The game seeks to provide players with practical experience of the communication and collaboration needed among different stakeholders to prepare for and respond to water contamination emergencies. Initial findings from game sessions with 41 participants suggest that frequent, proactive, and transparent communication can expedite the decision-making process and resolve the crisis more effectively. The game results also reveal challenges in inter-organizational coordination and communication, highlighting the need for training and standardized communication terminologies and protocols. 

Mathematics serves as the cornerstone of every STEM major, and proficiency in this subject is paramount for achieving academic success, societal integration, and meaningful participation in the workplace. However, numerous students encounter challenges in mastering mathematical concepts. In West Virginia, students face an additional hurdle, poverty, with households in the state ranking among the lowest earners in the nation. This can have significant implications for the funding and resources available to the West Virginia School System. Recognizing the interplay between socioeconomic factors and academic performance is crucial for devising targeted interventions and providing necessary support. In pursuit of this understanding, during the 2023-2024 school year, we administered a survey in 50 STEM courses at Fairmont State University (n = 447) and collected data from the LEAD Center on tutoring. Preliminary results show that math made up 50% and chemistry 37% of requested tutoring subjects in STEM and 69.7% receiving tutoring were in their first two years. Survey demographics are the following: gender identity: 212 male, 212 female, and 4 non-binary; economic status: 82% were middle class, 13% working class, and 4% on assistance; 29.5% are first generation; 62% are within their first two years of study. The most common math completed in high school was algebra (24.8%), trigonometry (34%), and calculus (24.4%). There was a strong correlation between major and enjoying math (0.99) and having a good math teacher in high school (0.62). Through data analysis and working with students directly, our goal is to discover why students struggle with math and how to better prepare future generations.   

We report the first record of imposex in Triplofusus giganteus, a top predator in shallow water marine habitats. Imposex is an irreversible disorder in female caenogastropod snails caused by exposure to biocidal organotin compounds identified in vessel antifouling paints. The condition was observed in three of four snails collected from Alligator Harbor, Florida, with affected females developing a non-functional male reproductive organ, the pseudopenis. The presence of imposex is of particular concern for the long-term conservation of the species, given its slow maturation and limited reproductive output. 

ABSTRACT Many bacterial histidine kinases work in two-component systems that combine into larger multi-kinase networks. NahK is one of the kinases in the GacS Multi-Kinase Network (MKN), which is the MKN that controls biofilm regulation in the opportunistic pathogenPseudomonas aeruginosa. This network has also been associated with regulating many virulence factorsP. aeruginosasecretes to cause disease. However, the individual role of each kinase is unknown. In this study, we identify NahK as a novel regulator of the phenazine pyocyanin (PYO). Deletion ofnahKleads to a fourfold increase in PYO production, almost exclusively through upregulation of phenazine operon two (phz2). We determined that this upregulation is due to mis-regulation of allP. aeruginosaquorum-sensing (QS) systems, with a large upregulation of thePseudomonasquinolone signal system and a decrease in production of the acyl-homoserine lactone-producing system,las. In addition, we see differences in expression of quorum-sensing inhibitor proteins that align with these changes. Together, these data contribute to understanding how the GacS MKN modulates QS and virulence and suggest a mechanism for cell density-independent regulation of quorum sensing. IMPORTANCEPseudomonas aeruginosais a Gram-negative bacterium that establishes biofilms as part of its pathogenicity.P. aeruginosainfections are associated with nosocomial infections. As the prevalence of multi-drug-resistantP. aeruginosaincreases, it is essential to understand underlying virulence molecular mechanisms. Histidine kinase NahK is one of several kinases inP. aeruginosaimplicated in biofilm formation and dispersal. Previous work has shown that the nitric oxide sensor, NosP, triggers biofilm dispersal by inhibiting NahK. The data presented here demonstrate that NahK plays additional important roles in theP. aeruginosalifestyle, including regulating bacterial communication mechanisms such as quorum sensing. These effects have larger implications in infection as they affect toxin production and virulence. 

I present the largest survey of seed germination yet for Barro Colorado Island (BCI) and central Panama, based on 1,226 seed collections from 732 species, in 103 families, including trees, shrubs, lianas, vines, herbs, and epiphytes. Most collections were germinated in both sun and shade habitats in growing houses on BCI, simulating tree fall gap and shaded understory conditions. Many ad hoc treatments were also utilized. Data were collected during the field portion (1985–1989) of the BCI Seedling Flora Project. This publication marks the public release of the entire dataset, parts of which have previously been shared with BCI colleagues. I hope that this dataset will provide background information for those needing to produce seedlings from seed for experimental studies and that it will encourage others to incorporate seed germination as an additional plant trait into community analyses. 

Abstract College science programs exhibit high rates of student attrition, especially among Students of Color, women, members of the LGBTQ+ community, and those with disabilities. Many of the reasons students choose to leave or feel pushed out of science can be mitigated through participation in faculty-mentored research. However, faculty resources are limited, and not every student has access to faculty mentoring due to systemic or structural barriers. By bringing authentic scientific research into the classroom context, course-based undergraduate research experiences (CUREs) expand the number of students who participate in research and provide benefits similar to faculty-mentored research. Instructors also benefit from teaching CUREs. Using a systematic review of 14 manuscripts concerning the Malate Dehydrogenase CUREs Community (MCC) and malate dehydrogenase (MDH) CUREs, we demonstrate that CUREs can be implemented flexibly, are authentic research experiences, generate new scientific discoveries, and improve student outcomes. Additionally, CURE communities offer substantial advantages to faculty wishing to implement CUREs. 

This review discusses the intriguing yet controversial concept of metabolons, focusing on the malate dehydrogenase-citrate synthase (MDH-CISY) metabolon as a model. Metabolons are multienzyme complexes composed of enzymes that catalyze sequential reactions in metabolic pathways. Metabolons have been proposed to enhance metabolic pathway efficiency by facilitating substrate channeling. However, there is skepticism about the presence of metabolons and their functionality in physiological conditions in vivo. We address the skepticism by reviewing compelling evidence supporting the existence of the MDH-CISY metabolon and highlighting its potential functions in cellular metabolism. The electrostatic interaction between MDH and CISY and the intermediate oxaloacetate, channeled within the metabolon, has been demonstrated using various experimental techniques, including protein–protein interaction assays, isotope dilution studies, and enzyme coupling assays. Regardless of the wealth of in vitro evidence, further validation is required to elucidate the functionality of MDH-CISY metabolons in living systems using advanced structural and spatial analysis techniques.